free download of microsoft access sample database

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If you are the author of this article, you do not need to request permission to reproduce figuresĪnd diagrams provided correct acknowledgement is given. Provided correct acknowledgement is given. If you are an author contributing to an RSC publication, you do not need to request permission To request permission to reproduce material from this article, please go to the Ultrasensitive Amplification-free Detection of Circulating miRNA via Droplet-based Processing of SERS tag–miRNA–magnetic Nanoparticle Sandwich Nanocomplex on Paper-based Electrowetting-on-dielectric Platform The versatility of this method needless of RNA extraction for clinical samples is expected to have a good potential in detecting other miRNAs. The detection limit of this approach can reach 0.26 fM through SERS detection. The pEWOD device was used to achieve short cleanup time and good recovery of the nanocomplex, bringing the total analysis time to be less than 30 min. Then the target analyte miRNA-200a-3p was introduced to form a sandwich nanocomplex through hybridization with the pair of LNA probes. A sandwich approach where NAEBs modified by one LNA-1 probe and MNPs modified by another LNA-2 probe was applied. In this study, hsa-miR-200a-3p of the miR-200a sub-family, which is a biomarker of ovarian cancer, was used as the analyte to demonstrate the analytical capability of an integrated biosensing platform using an extremely sensitive surface enhanced Raman scattering (SERS) nanotag – nanoaggregate-embedded beads (NAEBs), magnetic nanoparticles (MNPs), a pair of highly specific locked nucleic acid (LNA) probes, and a semi-automated paper-based electrowetting-on-dielectric (pEWOD) device to provide the labor-less and thorough sample cleanup and recovery. However, quantifying miRNAs in human blood samples is challenging owning to issues of sensitivity and specificity. MicroRNAs (miRNAs) have emerged as a promising class of biomarkers for early detection of various cancers, including ovarian cancer.

Free download of microsoft access sample database